Gilson Connect
This application note demonstrates the use of EXTRACTMAX™ for fully automated magnetic bead–based purification of proteins, with a focus on preserving weak and transient protein–protein interactions. Using Gilson’s exclusion-based sample preparation (ESP™) technology, EXTRACTMAX gently transfers magnetic beads through multiple wash steps without centrifugation or harsh aspiration. The study highlights reproducible protein recovery, minimal sample loss, and parallel processing of up to four samples, illustrating how EXTRACTMAX supports reliable and automated magnetic purification workflows.
Get fast, high-yield, and high-purity isolations without the need for long washing steps. This is possible thanks to our exclusive Exclusion-based Sample Preparation (ESP™) technology. It is perfect for capturing weakly bound protein complexes without subjecting them to high shear forces from pipette-based wash steps. Plus, it prevents contamination, dilution, or discarding of the original samples.
For labs looking to uncover weak protein interactions, conventional methods aren’t ideal. Consider exclusion-based sample preparation from EXTRACTMAN instead.
Magnetic bead extraction process can help improve your protein isolation workflows and speed up the efficiency of both IP and Co-IP lab procedures.
Josh Martin, a graduate research assistant and Ph.D. candidate with the McCardle Laboratory for Cancer Research, discusses how EXTRACTMAN helps to overcome limitations of the immunoprecipitation techniques that aim to identify and isolate binding partners of proteins.
Protein-protein interactions play an ever important role in oncogenesis. In this video, Dr. Richard Burgess, Professor Emeritus of Oncology at the University of Wisconsin, discusses approaches to characterize weak protein interactions that are targeted by cancer researchers.
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has been used to create useful animal models for studying complex diseases, as well as correcting or inducing disease states within a cell. This technical note describes how PLATEMASTER and EXTRACTMAN can be used in the rapid enrichment and clonal selection of CRISPR modified cells.
This application note presents a new approach for the cleanup of DNA fragments from small volumes of liquid combining the innovative design of EXTRACTMAN® with the convenience of a DNA purification using MACHEREY-NAGEL’s proven NucleoMag® technology.
RNA-binding proteins (RBPs) are involved in many key cellular processes, but identification of the native binding partners (target RNAs) can be challenging. In this application note, we show that EXTRACTMAN can be used to efficiently and specifically enrich endogenously expressed ribonucleoprotein complexes.
EXTRACTMAN® improved isolation speed nine-fold and produced equivalent or better yields of purified DNA compared to tube-based isolation methods. In this application note, four DNA samples from two different organisms (mammalian and plant) were processed in parallel with no cross-contamination.
