Automation of a Secondary Drug Screen for ALS Using iPSC-Derived Human Neurons

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease caused by the death of motor neurons. A hallmark of ALS is low expression of neurofilament light chain (NFL) in motor neurons. A high-throughput phenotypic assay using patient-derived induced pluripotent stem cells (iPSCs) was used to screen over 6,000 compounds. This poster demonstrates how automation of serial dilutions and dosing on PIPETMAX 268 provides a more accurate and less labor-intensive alternative to manual pipetting for rescreening.