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Application Notes
Sample preparation for any form of analysis can be tedious and time-consuming, and efforts to automate this process have been long and fruitful. Today, many different types of liquid handlers exist that can perform a variety of functions to increase throughput tremendously. In this application note, we demonstrate one example of sample prep by taking a common but complicated screening method for optimizing liquid-liquid chromatography run parameters and automating the process. We also showcase the use of liquid level detection in the precise liquid handling of biphasic solutions
Artemisinin is a sesquiterpene lactone obtained from sweet wormwood, Artemisia Annua L. The extract of the leaves of Artemisia Annua L. is used in ancient Chinese herbal therapy for malarial fevers. Artemisinin has been identified as an active antimalarial compound. In this application note, the use of CPC coupled with a preparative liquid chromatography system equipped with UV-DAD and mass spectrometry (MS) detectors, illustrates the success of such technologies to isolate active compounds from complex mixtures.
Piperine, an alkaloid that gives black pepper its pungent taste (Piper nigrum), is also associated with several bioactivities and can be used as a spray in personal defense devices. The preparative separation and purification of piperine is usually performed by conventional methods. Among those methods are centrifugal partition chromatography (CPC) and flash chromatography. In this study, a crude extract of black pepper is used to purify piperine and to compare the performance of CPC versus flash chromatography - read more about the methods and results in this application note.
In recent years, kratom has become an increasingly popular recreational drug around the world; consequently, this increase in popularity is matched by increasing concern over its potential to worsen the current opioid epidemic. As a result, further studies of kratom and its constituent compounds are necessary to better understand the mechanisms of action of these compounds and to evaluate possible therapeutic benefits of mitragynine or 7-hydroxymitragynine, present in kratom. Learn more in this application note.
Lignin is an abundant phenylpropanoid polymer found in all plant matter. It has historically been treated as an impurity in the paper pulping and ethanol biorefinery industries where it is burned as a low-value fuel or sold at low prices. However, developing higher-value applications of lignin would help biorefineries operate at higher efficiencies. In this app note, a two-dimensional separation is conducted using centrifugal partition chromatography (CPC), which helps demonstrate the value of CPC as a tool for gaining deeper insights into depolymerization mixtures for biomass facilities.
Forsythiaside A, a phenylethanoid glycoside, is one of the major active constituents extracted from the air-dried fruits of Forsythia suspensa and has been reported to possess a wide range of pharmacological properties, including anti-inflammatory and antioxidant activities. This application note discusses a simple, fast, and cost-efficient method to prepare pure compounds using a centrifugal partition chromatography (CPC) system.
Fermentation allows for the conversion of organic materials into relatively simple substances by using micro-organisms such as bacteria, Streptomyces, filamentous fungi, or yeast. In this app note, we demonstrate the added value of CPC at achieving an efficient separation from a fermentation broth in one step.
Accurately quantify THC and metabolites for forensic purposes
Ginger is widely known for its use in flavoring foods and beverages, but it is also commonly used for medicinal, perfumery, and esthetic purposes. This app note shows how CPC can produce a high-quality standard of [6]-gingerol without the use of silica-based chromatography methods, such as FLASH and prep HPLC.
CPC is recognized as the preferred technology for the purification of high quality Δ-9-tetrahydrocannabinol (THC) and cannabidiol (CBD) for the pharmaceutical and food supplement markets. The objective of this app note was to test the high selectivity of CPC and assess its capability to purify a set of key compounds (THC, CBD, CBN and CBG) and enrich several minor cannabinoids (CBDV, CBCV and CBC).